| Cell type (class) | Nomenclature (Acronym) |
Definition | Relation of burst LTS neuron |
| Annotation | Reference |
Collator |
| |
| descending neuron | Swanson (Swanson) | Collator note: those neurons that project to the autonomic centers located in the spinal cord and in the dorsal medulla, and are located in the hypothalamus. | partially corresponds | | Approximately 75% of cells that were negative for fluoro-gold displayed an LTS and expressed a relatively large T-current. This finding is consistent with that of a recent study reporting that PVN preautonomic neurones backfilled by tracer injection in the brainstem expressed an LTS (10). | Luther J.A., Daftary S.S., Boudaba C., Gould G.C., Halmos K.CS. & Taker J.G. | Mihail Bota |
|
| PVN pre-autonomic type A neuron | Stern (Stern) | PVN preautonomic type A neurones were located exclusively in the PaV subnucleus and were the most common cell type labelled, accounting for 52 % of all labelled neurones in the PVN (Fig. 3A). They had a mean cross-sectional soma area of 134.8 ± 15.4 µm2. Dendrites were often varicose, and short spinous processes were occasionally observed. A
common observation (also found for the other neuronal
types) was that distal dendritic branches extended
beyond the boundaries of the subnucleus and tended to
approach the walls of the 3V. A morphometric analysis
of reconstructed neurones showed that type A neurones
(n = 33) had 2.7 ± 0.1 primary dendrites, which gave rise
to 6.4 ± 0.7 branches. The TDL, MDL and mean path
length (MPL) were 2093.7 ± 215.8 µm, 766.6 ± 91.4 µm
and 399.7 ± 33.2 µm, respectively (see Fig. 4, for
comparison with the other neuronal types). Axons were
identified by their thinner diameter and beaded
appearance...axons [...] could be traced for several hundred
micrometres, running laterally or ventrolaterally towards the lateral hypothalamic area. In 25/33 (78 %) type A
neurones, axons arose from a primary dendrite at a mean
distance from the soma of 41± 5 µm (Fig. 3A1 and A2).
In the remaining type A neurones, axons arose directly
from the soma. | partially corresponds | | LTSs with variable shapes and amplitudes, ranging from small-amplitude'humps' to long-lasting plateaus, were observed in 35/41 (84%) type A, 20/20 (100%) type B and 15/18 (85%) type C neurones. The incidence of LTSs was not significantly different among neuronal types. | Stern J.E. | Mihail Bota |
|
| PVN pre-autonomic type B neuron | Stern (Stern) | PVN preautonomic type B neurones were located exclusively in the PaPo subnucleus, and accounted for 25 % of the recorded neurones (Fig. 3B). They had a mean crosssectional soma area of 188.7 ± 4.2 µm2, which was not significantly different from the other PVN pre-autonomic neurones (P > 0.5, one-way ANOVA). A morphometric analysis of reconstructed neurones revealed that type B pre-autonomic neurones (n = 15) had the most complex dendritic arborization, as compared to the other neuronal
types. Type B neurones had 3.8 ± 0.1 primary dendrites, which gave rise to 10.7 ± 1.9 branches (both parameters were significantly larger than the other neuronal types, P < 0.001 and P < 0.02, respectively, one-way ANOVA, Fig. 4). The TDL was also significantly larger than in the other neuronal types (5329.3 ± 1500 µm, P < 0.02, oneway ANOVA, Fig. 4). The MDL and MPL were 778.6 ± 310.7 µm and 390.5 ± 104.4 µm, respectively (not different from the other neuronal types). Similar to type A neurones, in the majority of cases (12/15, 80 %) axons arose from a primary dendrite, at a mean distance from the soma of 38 ± 6 µm. | partially corresponds | | LTSs with variable shapes and amplitudes, ranging from small-amplitude'humps' to long-lasting plateaus, were observed in 35/41 (84%) type A, 20/20 (100%) type B and 15/18 (85%) type C neurones. The incidence of LTSs was not significantly different among neuronal types. | Stern J.E. | Mihail Bota |
|
| PVN pre-autonomic type C neuron | Stern (Stern) | PVN preautonomic type C neurones accounted for 23% of recorded neurones, with the great majority of them (83 %) located in the PaPo subnucleus (Fig. 3C). They had a mean cross-sectional soma area of 165.4 ± 40 µm2, with 3.1± 0.2 primary dendrites, which gave rise to 7.9 ± 0.9 branches (n = 17). Similar to the other neuronal types, dendrites were often varicose and tended to approach the walls of the 3V. In two cases, dendrites of type C neurones were observed to cross to the contralateral
PVN (see Fig. 1D). The TDL, MDL and MPL of type C neurones were 2168.2 ± 750 µm, 692.9 ± 220 µm and 348.5 ± 86 µm, respectively. In contrast to type A and B neurones, in the majority of type C neurones (10/17, 60%), axons arose from the soma (Fig. 3C1 and C2). However, the incidence of axon origin was not significantly different between cell types (P = 0.1, x2 test). | partially corresponds | | LTSs with variable shapes and amplitudes, ranging from small-amplitude'humps' to long-lasting plateaus, were observed in 35/41 (84%) type A, 20/20 (100%) type B and 15/18 (85%) type C neurones. The incidence of LTSs was not significantly different among neuronal types. | Stern J.E. | Mihail Bota |
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