| Staining type: thionin |
Sampling: 1:5 |
Annotation: Up to five one-in-five series of 20 micrometers thick frozen sections through the PVH were saved and then prepared for indirect immunofluorescence staining of cells that cross-react with antisera against CRF, neurotensin, oxytocin, or vasopressin using a conventional
method (Sawchenko and Swanson, 1981) based upon the localization of a primary antiserum raised in rabbits using an affinity-purified, fluorescein-conjugated goat anti-rabbit IgG. The distributions of labeled cells were mapped onto projection drawings made from adjacent series of sections counterstained with thionin, and the region around the PVH was photographed at x 100 magnification using Ilford XP-1 film. |
