Metadata

Experiment acronym Annotation Collator Author checked
Broberger-H/O-MCHIn situ hybridization study of the H/O and MCH neurons distributions in the rat hypothalamusMihail Botano

Animals (subjects)
Sex: M
Number of animals: 10
Age: not specified
Mass: 250-300
Unit of mass:
Housing conditions: normal housing
Annotation: Male Sprague-Dawley rats (n = 10, 250–300 g; B&K Universal, Stockholm, Sweden) and male C57B1mice (n = 5, 20–40 g; B&K Universal) were used. The animals were kept under regular lighting conditions (lights on at 6.00 and off at 18.00) in a temperature-controlled environment and had free access to standard rodent chow and tap water.
Experimental method
Experiment type: in situ hybridization
Neuron/glia identification method: stain specific
Staining frequency: 1:10

Experimental details:
Measured nucleic acid: mRNA
Source (producer): Scandinavian Gene Synthesis, Koping, Sweden).
Probe sequence: 2-49 H/O nucleotides
Sequence species: rat
Probe sequence orientation: antisense
Control: sense
Labelling method: radiolabelling
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Probes complementary to nucleotides 479-527 of the rat MCH mRNA(Nahon et al., 1989) and to nucleotides 2-49 of the rat H/O mRNA(Sakurai et al., 1998) were synthesized (Scandinavian Gene Synthesis, Ko¨ping, Sweden). Probe sequences were controlled against other sequences in the GenBank database, and no homologies exceeding 75% were found.

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 14 micrometer
Staining type: toluidine blue Sampling: 1:10 Annotation: The brains were cut serially at 14micrometers thickness on a cryostat (Microm, Heidelberg, Germany), and every tenth section was processed for double-labeling in situ hybridization.

Mapping details
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