| Animals (subjects) | | Sex: M | | Number of animals: not specified | | Age: not specified | | Mass: 225-250 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: Adult male Sprague Dawley rats (225-250 gm body weight at the beginning of the experiment) were maintained on a 12 hr light/dark photoperiod (lights on at 6:00 AM) with ad libitum access to water and rat chow and were allowed at least 5d of acclimation to the animal quarters. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: stain specific | | Staining frequency: 1:8 |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: preproCRH
Sequence species: not specified
Probe sequence orientation: antisense
Control: not specified
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: film
Annotation: Sections were hybridized with 35S-UTP-labeled cRNA probes transcribed from cDNA sequences encoding RNAs for parts of the following genes: preproCRH (700 bp)...
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