| Animals (subjects) | | Sex: M | | Number of animals: 5 | | Age: not specified | | Mass: 280-320 | | Unit of mass: | | Housing conditions: normal housing | | Annotation: Adult male Sprague-Dawley rats (280-320 g BW at injection) were maintained on a 12 hour light/ 12 hour dark photoperiod (lights on 0700 hours) with water and rat chow available ad libitum. They were allowed 7 days' acclimatization to the animal quarters before we proceeded with the experiment. |
| | Experimental method | | Experiment type: in situ hybridization | | Neuron/glia identification method: stain specific | | Staining frequency: 1:8 |
Experimental details:
| Measured nucleic acid: mRNA
Source (producer): Promega Gemini
Probe sequence: pp-neurotensin/neuromedin
Sequence species: not specified
Probe sequence orientation: antisense
Control: sense
Labelling method: not specified
Visualization method: autoradiogram
Visualization medium: slide
Annotation: Sections were hybridized with 35S UTP- labelled complementary RNA (cRNA) probes transcribed from cDNA sequences for part of the mRNAs encoding pp-neurotensin/neuromedin N (NT/N), or pp-vasopressin...Details of the probes and their
characterization have been given in publications from this and other laboratories (Carrazana et al., 1988; Swanson and Simmons, 1989; Frim et al., 1990; Watts, 1992b).. Additional experiments (data not given) have shown that neither RNase pretreatment followed by hybridization with antisense strand probes nor hybridization with sense strand probes gives any hybridization...All 35S-UTP probes were synthesized using the Riboprobe Gemini System I1 kit (Promega Inc., Madison, WI) and the appropriate RNA polymerase using a modification of the method of Melton et al. (1984)
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