| Animals (subjects) | | Sex: M | | Number of animals: 10 | | Age: not specified | | Mass: not specified | | Unit of mass: not specified | | Housing conditions: normal housing | | Annotation: The second group consisted of animals that received a single injection of colchine (4-8 microgram/microliter) saline into either the lateral ventricle (100-200 micrograms; n=8) or the fourth ventricle (50 micrograms; n=2) the day before perfusion |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: not specified | | Staining frequency: 1:4 or 1:8 | 8, 2
Experimental details:
| Antigen: not specified
Antigen species: not specified
Source (producer): not specified
Primary antibody: not specified
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): polyclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: not specified
Visualization method: fluorescence
Visualization medium: slide
Annotation: Seven different antisera to synthetic ovine CRF (or to a fragment of CRF), which were raised in rabbits [7], were tested and all but one were found to stain cells and fibers in the rat. Since antiserum 24 ([Try22. Gly23]-CRF (1-23) conjugated to human alpha-globulin with bis-diazetized benzidine] has been found to interact with rat CRF particularly well in radioimmunoassays [Vale, unpublished observations], it was used extensively for the mapping studies reported here.
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