| Animals (subjects) | | Sex: M | | Number of animals: 9 | | Age: not specified | | Mass: not specified | | Unit of mass: not specified | | Housing conditions: normal housing | | Annotation: A total of 30 adult male rats, consisting of 4 different groups was used. The first was made up of 13 normal male Sprague-Dawley rats. 5 of them received colchicine into the lateral cerebral ventricle, 4 received intracisternal colchicine. |
| | Experimental method | | Experiment type: immunohistochemistry | | Neuron/glia identification method: stain specific | | Staining frequency: 1:5 or 1:10 | 30, 2000
Experimental details:
| Antigen: AII
Antigen species: not specified
Source (producer): specified elsewhere
Primary antibody: Rabbit DE
Primary antibody species: rabbit
Antibody type (monoclonal, polyclonal): not specified
Secondary antibody: not specified
Secondary antibody species: goat
Immunoglobulin class: Alpa globulin
Control: adsorption
Visualization method: fluorescence
Visualization medium: slide
Annotation: The production and characterization of the AII antiserum (Rabbit DE) are described in detail elsewhere [8,58]. For all of our experiments it was used at a dilution of 1:2,500 and the secondary antiserum, affinity-purified goat-anti-rabbit conjugated to fluorescein isothycyanate (Tago), was used at a dilution of 1:200. To demonstrate specificity of immunohistochemical staining, all of the major cell groups and fiber systems described below were examined with anitserum that was blocked by liquid-phase absorption. The same solid-pahse adsorption technique was used to demonstrate an absence of crossreactivity between the anti-AII serum and vasopressin in the hypothalamus and basal telencephalon.
| |
|
