Metadata

Experiment acronym Annotation Collator Author checked
Ojima-CHATImmuhistochemical study of CHAT presence in the rat cerebellum.Mihail Botano

Animals (subjects)
Sex: M
Number of animals: 14
Age: not specified
Mass: 175-227
Unit of mass:
Housing conditions: not specified
Annotation: A total of 14 male rats (Std:Wistar; Shizuoka Lab. Animal, Japan), weighing 175 to 227 g were used for the immunohistochemical investigation. Additionally, two normal rats were sacrificed for comparable histological analysis by staining preparations with 0.1 5% thionin (Sigma).
20, 3670
Experimental method
Experiment type: immunohistochemistry
Neuron/glia identification method: morphology
Staining frequency: 1:5

Experimental details:
Antigen: CHAT
Antigen species: hog
Source (producer): Boehringer Mannheim GmbH)
Primary antibody: antiCHAT antibody
Primary antibody species: not specified
Antibody type (monoclonal, polyclonal): monoclonal
Secondary antibody: not specified
Secondary antibody species: not specified
Immunoglobulin class: not specified
Control: staining pattern
Visualization method: colorimetry
Visualization medium: slide
Annotation: The primary antibody employed in the present study was a monoclonal antibody raised against ChAT from hog brain (Boehringer Mannheim GmbH); its specificity has been well characterized (Eckenstein and Thoenen, '82). The antibody has been used for immunohistochemical localization of cholinergic elements in various species (Eckenstein and Sofroniew, '83; Carlsen and Heimer, '86; Rao et al., '87; Stichel and Singer, '87; Ojima et al., '88a and others). .. immunohistochemical staining with the unlabeled antibody peroxidase-antiperoxidase (PAP) method (Sternberger, '79).

Anatomy and histology
Section plane: coronalAngle: not specifiedCutting method: microtome Preservation: freezing Thickness: 40 micrometer
Staining type: thionin Sampling: 1:5 Annotation: All sections from six sagittally cut cerebella and every fifth section from five sagittally and three coronally cut cerebella were subjected, in a free-floating manner, to immunohistochemical staining with the unlabeled antibody peroxidase-antiperoxidase (PAP) method (Sternberger, '79). Some sections were counterstained with 0.1 % thionin.

Mapping details
Coordinates: noneData presentation:
text
representative labeled images
representative images with label and drawn boundaries
representative drawings or mappings onto templates
Mapping approach: brain region is captured in a BAMS nomenclature other than that used in the original publication